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目的：探索胃泌素（Ｇａｓｔｒｉｎ）对胆管癌细胞增殖的调节作用。方法：应用细胞培养技术及３Ｈ－Ｔｄｒ掺入法，研究了胃泌素－１７（Ｇ－１７）、亮氨酸胃泌素－１７（ＬｅｕＧ－１７）、五肽胃泌素（Ｇ－５）及胆囊收缩素（ＣＣＫ－Ｂ／Ｇａｓｔｒｉｎ）受体拮抗剂Ｌ－３６５，２６０（Ｌ－６０）对培养的人胆管癌细胞ＱＢＣ９３９（ＱＢ）、ＯＣＵＣｈ－ＬＭ１（ＯＣ）、ＨｕＣＣ－Ｔ１（Ｈｕ）、ＯＺ增殖的影响。结果：Ｇ－１７、ＬｅｕＧ－１７、Ｇ－５通过ＣＣＫ－Ｂ／Ｇａｓｔｒｉｎ受体显著的促进了ＱＢ、ＯＣ的增殖，而对Ｈｕ和ＯＺ的增殖无明显影响。结论：部分胆管癌细胞有ＣＣＫ－Ｂ／Ｇａｓｔｒｉｎ受体表达，胃泌素可促进胆管癌细胞的增殖，为发展胆管癌的生物学治疗方式提供了新的资料。
Objective: To explore the regulatory effect of gastrin on the proliferation of cholangiocarcinoma cells. METHODS: Gastrin-17 (G-17), Leucine-17 (LeuG-17), pentagastrin (G-5) were studied by cell culture and 3H-Tdr incorporation. Cholecystokinin (CCK-B/Gastrin) Receptor Antagonist L-365,260 (L-60) Against Cultured Human Cholangiocarcinoma Cells QBC939 (QB), OCUCh-LM1 (OC), HuCC-T1 (Hu) ), OZ proliferation effects. RESULTS: G-17, LeuG-17 and G-5 significantly promoted the proliferation of QB and OC through the CCK-B/Gastrin receptor, but had no significant effect on the proliferation of Hu and OZ. Conclusion: Some of the cholangiocarcinoma cells have CCK-B/Gastrin receptor expression, gastrin can promote the proliferation of cholangiocarcinoma cells, and provide new information for the development of biological treatment of cholangiocarcinoma.