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目的研究丹参酮ⅡA磺酸钠对高脂性脂肪肝(HFL)大鼠肝脏脂质过氧化的影响,并初步探讨其作用机制。方法用高脂饲料诱发大鼠HFL模型,8周后将建模成功的大鼠随机分为模型组、丹参酮ⅡA磺酸钠(大、小剂量组)和阳性药易善复(PPC)组,每组均给予高脂饲料及相应药物,正常对照组喂以基础饲料。连续给药6周后比较各组间肝指数、血脂、血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(ASL)活性和超氧化物歧化酶(SOD)、丙二醛(MDA)的含量;检测肝脂质、肝脏组织中SOD、MDA含量并进行肝脏病理学检查。结果丹参酮ⅡA磺酸钠可显著降低HFL模型大鼠肝指数,降低血清和肝组织中TG、TC、MDA水平,降低血清LDL-C含量和ALT、AST活性;增加血清和肝组织中SOD活性及HDL-C含量,与模型组相比差异显著(P<0.05或<0.01);并可明显减少肝细胞浆内脂滴分布的程度和炎症范围。结论丹参酮ⅡA磺酸钠可通过改善肝功能及脂质代谢紊乱,减少肝中脂质沉积,减轻肝组织损伤,对高脂性脂肪肝有一定的治疗作用。
Objective To study the effect of sodium tanshinone IIA sulfonate on hepatic lipid peroxidation in rats with high fat fatty liver (HFL), and to explore its mechanism. Methods The HFL model was induced by high-fat diet. Eight weeks later, the successfully modeled rats were randomly divided into model group, tanshinone IIA sulfonate (large and small dose group) and positive drug Yishanfu (PPC) group. Each group was given high-fat diet and corresponding drugs, and the normal control group was fed with basal diet. After 6 weeks continuous administration, liver index, blood lipids, serum alanine aminotransferase (ALT), aspartate aminotransferase (ASL) activity and superoxide dismutase (SOD), malondialdehyde (MDA) were compared among groups. Content; Detection of hepatic lipids, liver tissue SOD, MDA content and liver pathological examination. RESULTS: Sodium tanshinone IIA sulphate can significantly reduce the hepatic index of HFL model rats, decrease the levels of TG, TC, MDA in serum and liver tissue, decrease the serum LDL-C content and ALT, AST activity, and increase the activity of SOD in serum and liver tissue. The HDL-C content was significantly different from that in the model group (P<0.05 or <0.01), and significantly reduced the degree of lipid droplet distribution and inflammation in the hepatocyte cytoplasm. Conclusion Sodium tanshinone II A sulfonate can improve liver function and lipid metabolism, reduce lipid deposition in the liver, and reduce liver injury. It has a certain therapeutic effect on fatty liver.