论文部分内容阅读
背景:阿尔茨海默病(Alzheimerdisease,AD)的病理病因虽然还没有完全清楚,但目前认为β-淀粉样肽(amyloidβ-peptide,Aβ)是引起AD的胆碱能神经功能紊乱的主要成分。因此,Aβ的产生及其对神经细胞的影响,以及寻找该病的发病机制、治疗方法及其有效的治疗药物成了医药界的研究热点。目的:观察三七总皂甙(PNS)对NG108-15细胞老年性痴呆模型的影响,为寻找该病的发病机制提供佐证,为研究和开发中药的新成分提供理论依据。设计:体外平行对照实验。单位:广西中医学院新药开发中心,广西中医学院生物化学教研室。对象:实验在广西中医学院新药开发中心完成。NG108-15细胞:中国科学院上海细胞研究所提供。PNS:云南玉溪维和制药厂生产。Aβ25～35片段:德国Sigma公司产品,批号:042K49501。MTT(四甲基噻唑蓝),德国Sigma公司产品,批号:020609。cAMP(环一磷酸腺苷)日本ャマサ酱油株式会社产品,批号:00207。胚胎牛血清(FBS):由美国Hy-clone公司提供,批号:0405。DMEM培养基:美国GIBCO公司产品,批号:0311。干预:NG108-15细胞培养法:含50mL/L胚牛血清、10g/LHAT、10g/L青霉素和链霉素(1:1)的DMEM培养液,置100mL/LCO2、37℃用的培养箱内培养殖,每隔一天换一次液,4d传代一次。主要观察指标:利用MTT法、细胞计数法和细胞形态学?
BACKGROUND: Alzheimer disease (AD) pathological causes have not yet been fully understood, but it is currently considered that amyloid β-peptide (Aβ) is a major component of cholinergic nerve dysfunction that causes AD. Therefore, the production of Aβ and its effects on nerve cells, as well as the search for the pathogenesis of the disease, treatment methods and effective therapeutic drugs have become a research hotspot in the medical community. OBJECTIVE: To observe the effect of panax notoginseng saponins (PNS) on senile dementia in NG108-15 cells, provide evidence for the pathogenesis of the disease, and provide theoretical basis for the research and development of new components of traditional Chinese medicine. Design: In vitro parallel control experiment. Unit: New Drug Development Center, Guangxi Traditional Chinese Medical College, Department of Biochemistry, Guangxi University of Traditional Chinese Medicine. Subject: The experiment was completed at the New Drug Development Center of Guangxi College of Traditional Chinese Medicine. NG108-15 cells: Shanghai Institute of Cellular Sciences, Chinese Academy of Sciences. PNS: Yunnan Yuxi Pharmaceutical Factory production. Fragments of Aβ25-35: Sigma, Germany, batch number: 042K49501. MTT (tetramethylthiazole blue), Sigma, Germany, lot number: 020609. cAMP (cyclic adenosine monophosphate) Japanese wolfberry soy sauce Co., Ltd. lot number: 00207. Embryo bovine serum (FBS): supplied by Hy-Clone, USA, Lot 0405. DMEM medium: U.S. GIBCO product, batch number: 0311. Intervention: NG108-15 cell culture method: DMEM medium containing 50 mL/L embryo calf serum, 10 g/LHAT, 10 g/L penicillin, and streptomycin (1:1), and 100 mL/LCO2, 37°C incubator Internal culture, change the fluid every other day, 4d pass once. MAIN OUTCOME MEASURES: Using MTT assay, cell counting, and cell morphology?