目的：建立测定氧化苦参碱血药浓度的ＨＰＬＣ法。方法：血浆标本经碱化以氯仿一正丁醇（９８∶２）提取，吹干后以甲醇一正己烷（４∶１，氨水调ｐＨ至８）为流动相，经过Ｓｐｈｅｒｉｓｏｒｂ－ＳｉＯ２（５μｍ）柱分离后在紫外２２０ｎｍ处检测。结果：氧化苦参碱血浆浓度曲线范围１２～３６ｍｇ·Ｌ－１，最低检出限为０１ｍｇ·Ｌ－１（Ｓ／Ｎ＝２）。样品在１２～３６ｍｇ·Ｌ－１浓度时的平均回收率为９８３１％～１０９０％，日内和日间精密度的ＲＳＤ均小于４％。结论：此方法为氧化苦参碱的血药浓度测定及药代力动学研究提供了一种简便可行的方法。 Objective: To establish an HPLC method for the determination of oxymatrine concentration. Methods: The plasma samples were alkalized and extracted with chloroform-n-butanol (98: 2). After drying with a mobile phase of methanol-n-hexane (4: Column after UV 220nm detection. Results: The curve of plasma concentration of oxymatrine ranged from 1 to 36 mg · L-1, with the lowest detection limit of 01 mg · L-1 (S / N = 2). The average recoveries of the samples ranged from 9831% to 1090% at 12 ~ 36 mg · L-1, and the RSDs of intraday and interday precision were less than 4%. Conclusion: This method provides a simple and feasible method for the determination of oxymatrine and its pharmacokinetic study.