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近年来有许多关于低温保存丝虫微丝蚴和第三期幼虫的报告,但未见有低温保存成虫的报告。为进行人体盘尾丝虫治疗等的研究,本实验用液氮低温保存牛盘尾丝虫(Onchocerca gutturosa)获得成功,并可长期保存和运输活成虫。牛盘尾丝虫雄虫取自自然感染牛体颈韧带的结缔组织中,先培养于RPMI 1640的培养液中,其中含有100U/ml青霉素、100μg/ml链霉素和0.25μg/ml两性霉素B,以及10％灭活小牛血清(IFCS)。低温保存的方法分为两组:
In recent years, there have been many reports on the preservation of filarial microfilaria and third larvae at low temperatures, but no reports of cryopreservation of adult worms have been reported. In order to study the onchocerciasis treatment on the human body, Onchocerca gutturosa was successfully preserved in liquid nitrogen at low temperature, and could preserve and transport live adults for a long time. Bombyx mori were obtained from connective tissue naturally infected with bovine and cervical ligaments and were first cultured in RPMI 1640 medium containing 100 U / ml penicillin, 100 μg / ml streptomycin and 0.25 μg / ml amphotericin B, and 10% inactivated calf serum (IFCS). Methods for cryogenic preservation fall into two groups: