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目的:考察典型肝脏细胞色素氧化酶P450酶系(肝药酶)诱导剂苯巴比妥钠干预给药对生附子毒性的影响,评价生附子毒性表现过程中的CYP450因素。方法:(1)测定小鼠单独给予生附子水煎液后的LD50;(2)测定苯巴比妥钠干预3天后,小鼠给予生附子水煎液的LD50;(3)Wistar大鼠经苯巴比妥钠(PB)干预给药3天后,十二指肠给予生附子水煎液,以出现频发室性早搏为其心脏毒性指标,观察PB干预及未干预的大鼠给予生附子后频发室性早搏发生率、出现时间及恢复率情况。结果:(1)小鼠给予生附子水煎液的LD50值为7.15g/kg,95%可信限为6.19～8.29g/(kg·d);(2)苯巴比妥钠诱导后,小鼠给予生附子水煎液的LD50>12.08g/(kg·d);(3)与单用生附子水煎液相比,苯巴比妥钠诱导后可显著减少生附子水煎液致大鼠频发室性早搏的发生率(P<0.01),延长出现时间(P<0.01),缩短持续时间(P<0.01)。结论:肝药酶诱导剂可显著降低生附子的毒性。
Objective: To investigate the effects of typical hepatic cytochrome oxidase P450 enzyme (hepatoprotectase) inducer phenobarbital sodium on the toxicity of Cyperus rotenotrophos and to evaluate the CYP450 factor during the toxicity of Cyperus rotundus. Methods: (1) To determine the LD50 of mice treated with Scutellariae Radix decoction alone; (2) To determine the LD50 of mice treated with Scutellariae Radix by three days after the intervention of phenobarbital sodium; (3) After 3 days of phenobarbital sodium (PB) administration, duodenum was given decoction of raw Aconitum to make it appear the cardiotoxicity index of frequent premature ventricular contractions. The rats of PB intervention and non-intervention were given Shengfuzi Frequent occurrence of premature ventricular contractions incidence, timing and recovery rate. The results showed that: (1) The LD50 of mice treated with DHF was 7.15g / kg and 95% confidence interval was 6.19-8.29g / (kg · d). (2) After induced by phenobarbital, Mice given LD50> 12.08g / (kg · d); (3) compared with the single use of Aconitum decoction, phenobarbital sodium induction can significantly reduce the raw Aconitum decoction caused The incidence of frequent premature ventricular contractions in rats was prolonged (P <0.01) and shortened (P <0.01). Conclusion: Liver enzyme inducer can significantly reduce the toxicity of raw monkshood.