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目的评估淡色库蚊抗药性测定中容器类型对结果的影响,确定能否用一次性杯子代替玻璃烧杯进行实验,从而达到提高实验效率的目的。方法从野外采集淡色库蚊幼虫,用WHO推荐的幼虫浸渍法,分别测定在玻璃烧杯、一次性塑料杯或一次性纸杯3种容器类型下,淡色库蚊幼虫对溴氰菊酯、仲丁威和双硫磷的LC50及95%可信区间(95%CI),并比较其差异。结果在玻璃烧杯、一次性塑料杯和一次性纸杯依次作为实验容器的条件下,淡色库蚊幼虫对溴氰菊酯的LC50及95%CI分别为0.002 009(0.001 654~0.002 449)、0.003 858(0.003 062~0.005 224)和0.005 459(0.004 051~0.008 757)mg/L,玻璃杯与2种一次性杯子间的结果差异有统计学意义;对仲丁威的LC50及95%CI分别为0.3470(0.3059~0.3821)、0.2575(0.2118~0.3095)和0.4844(0.4460~0.5232)mg/L,玻璃杯与一次性塑料杯差异无统计学意义,与一次性纸杯差异有统计学意义;而对双硫磷的LC50及95%CI分别为0.000 955(0.000 816~0.001 094)、0.003 007(0.002 661~0.003 407)和0.003 830(0.003 450~0.004 256)mg/L,玻璃杯与2种一次性杯子间差异均有统计学意义。用不同杯子测定蚊幼虫对杀虫剂的敏感性,溴氰菊酯的测定结果受浓度的影响小于双硫磷;双硫磷浓度越高杯子的影响越小。结论在对蚊幼虫的抗药性测定中,玻璃杯是最佳选择,而一次性杯子可能因对杀虫剂有吸附作用而使测定的结果发生偏差,使用一次性杯子替代玻璃杯时应该特别注意现做评估。
Objective To evaluate the influence of container type on Culex pipiens pallens resistance test to determine whether the disposable glass can be used instead of glass beaker to improve the experimental efficiency. Methods Culex pipiens pallens larvae were collected from the wild and larvae immersion method recommended by the WHO was used to determine the effects of deltamethrin on the larvae of C. pipiens pallens against deltamethrin, And the LC50 and 95% confidence interval (95% CI) of diphtheria and compare their differences. Results LC50 and 95% CI of Culex pipiens pallens to deltamethrin were 0.002 009 (0.001 654 ~ 0.002 449) and 0.003 858 respectively in the order of glass beakers, disposable plastic cups and disposable cups as experimental containers. (0.003 062-0 0.005 224) and 0.005 459 (0.004 051-0.008 757) mg / L respectively. There was significant difference in the results between glass and two kinds of disposable cups. The LC50 and 95% CI of s- 0.3470 (0.3059-0.3821), 0.2575 (0.2118-0.3095) and 0.4844 (0.4460-0.5232) mg / L, respectively. There was no significant difference between the glass cup and disposable plastic cup, The LC50 and 95% CIs for parathion were 0.000955 (0.000 816 to 0.001 094), 0.003 007 (0.002 661 to 0.003 407) and 0.003 830 (0.003 450 to 0.004 256) mg / L, respectively. Differences between the cups were statistically significant. The sensitivity of mosquito larvae to insecticides was determined by different cups. The determination of deltamethrin was less affected by the concentration than that of the pesticides. The higher the concentration of diphtheria, the smaller the influence of the cup. Conclusion In the determination of drug resistance to mosquito larvae, the glass is the best choice, but the disposable cup may be due to the adsorption of insecticides to make the determination results deviate from the use of disposable cups should pay special attention to the glass Now do assessment.