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Aim:To investigate the effect of water soluble-ethanol precipitation fraction(AC-1)and alkaline extraction-isoelectric precipitation fraction(AC-2)from Antrodiacamphorata(Polyporaceae,Aphyllophorales)on lipopolysaccharide(LPS)-in-duced gene activation in mouse macrophages.Methods:The AC-1 and AC-2fractions were prepared,and their effects on LPS-induced gene expression weremonitored by Western blotting and RT-PCR.Results:Our results indicatedthatAC-2,but not AC-1 dose-dependently(50-200 mg/L)inhibited LPS-induced nitricoxide production as well as the protein and the mRNA expression of the induciblenitric oxide synthase(iNOS)gene.Neither AC-1 nor AC-2 inhibited LPS-inducedcyclooxygenase-2 gene expression.Using the cytokine array assay,it showedthat AC-2 also had the ability to inhibit LPS-induced the protein expression ofinterleukin(IL)-6,1L-10,the monocyte chemoattractant protein(MCP)-5,and regu-lated upon activation,normal T-cell expressed,and presumably secreted(RANTES).Like iNOS,AC-2 inhibiting LPS-induced IL-6 and IL-10 secretion resulted frominhibiting their mRNA expression.Conclusion:It was suggested that alkalineextraction-isoelectric precipitated the polysaccharide fraction of A camphorataand had the ability to inhibit LPS-induced iNOS,IL-6,IL-10,MCP-5,and RANTESexpression in mouse macrophages.
Aim: To investigate the effect of water soluble-ethanol precipitation fraction (AC-1) and alkaline extraction-isoelectric precipitation fractions (AC-2) from Antrodiacamphorata (Polyporaceae, Aphyllophorales) on lipopolysaccharide (LPS) macrophages. Methods: The AC-1 and AC-2 fractions were prepared, and their effects on LPS-induced gene expression were confirmed by Western blotting and RT-PCR. Results: Our results indicated that ACh-2, but not AC- 50-200 mg / L) inhibited LPS-induced nitricoxide production as well as the protein and the mRNA expression of the inducible nitric oxide synthase (iNOS) gene. Neither AC-1 nor AC-2 inhibited LPS-inducedcyclooxygenase-2 gene expression. Using the cytokine array assay, it showed that AC-2 also had the ability to inhibit LPS-induced the protein expression of interleukin (IL) -6, L- L- 10, the monocyte chemoattractant protein (MCP) -5, and regu- lated upon activation, normal T-cell expressed, and presumably secreted (RANTES) .Like iNOS, AC-2 Inhibition of LPS-induced IL-6 and IL-10 secretion resulted in inhibition of their mRNA expression. Confluence: It was suggested that alkaline extract-isoelectric precipitated the polysaccharide fraction of A camphorata and had the ability to inhibit LPS-induced iNOS, IL- 10, MCP-5, and RANTESexpression in mouse macrophages.