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以小麦（辽春９号）为试验材料，应用同位素１５Ｎ示踪方法，采用棕壤定位试验１４年后不同施肥处理的土壤，研究作物对氮的吸收及肥料氮的去向。结果表明：在长期单施化肥的土壤中，植株吸收氮量以ＮＰＫ＞ＮＰ＞Ｎ，ＣＫ；在长期施有机肥和化肥的处理中，吸氮量以Ｍ１ＮＰＫ＞Ｍ１ＮＰ＞Ｍ１Ｎ＞Ｍ１。不同时期作物吸氮量与产量均呈明显正相关（ｒ＝０．９４～０．９９）。单施氮肥处理氮肥利用率只有６．２％；其它施肥处理氮肥利用率在２８．２％～３９．７％，尤以ＮＰＫ和Ｍ１ＮＰＫ处理利用率最高。残留肥料氮主要以根系残留物以及矿物和微生物体固定态氮存在，残留率除单施氮肥处理较高以外，平均为２６．４％。
Wheat (Liaochun 9) was used as experimental material. The isotope 15N tracing method was used to study the nitrogen uptake by crops and the fate of fertilizer nitrogen using different soil fertilization treatments after 14 years of brown soil location experiment. The results showed that NPK> NP> N and CK were the main nitrogen uptake by plants in long-term application of chemical fertilizers; M1NPK> M1NP> M1N> M1 in long-term application of organic fertilizer and chemical fertilizer. There was a significant positive correlation between the amount of nitrogen uptake and yield in different periods (r = 0.94 ~ 0.99). Nitrogen fertilizer application alone was only 6.2%; other fertilizer application nitrogen utilization rate was 28.2% ~ 39.7%, especially NPK and M1NPK treatment and utilization of the highest. Residual nitrogen fertilizer mainly root exudates and minerals and microbial fixed nitrogen exists, the residual rate except for higher nitrogen fertilizer treatment alone, an average of 26.4%.