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目的观察黄芪对大鼠局灶性脑缺血再灌注后脑组织IL-1β含量及MPO活性的影响,探讨其对脑缺血再灌注损伤保护作用的机制。方法分别采用酶联免疫吸附法(ELISA)及分光光度计法测定各组大鼠脑组织IL-1β含量及MPO活性。结果模型组与假手术组比较,IL-1β含量及MPO活性显著升高;黄芪组与模型组比较,IL-1β含量及MPO活性显著降低。结论黄芪可通过降低缺血再灌注后大脑皮质的IL-1β含量及MPO活性,从而减轻炎症反应,发挥其脑保护作用。
Objective To observe the effect of Radix Astragali on IL-1β and MPO activity in rat brain after focal cerebral ischemia and reperfusion, and to explore its protective mechanism against cerebral ischemia-reperfusion injury. Methods The levels of IL-1β and MPO activity in rat brain were determined by enzyme-linked immunosorbent assay (ELISA) and spectrophotometry, respectively. Results Compared with the sham-operated group, IL-1β content and MPO activity were significantly increased in the model group. Compared with the model group, IL-1β content and MPO activity were significantly decreased in the Astragalus group. Conclusion Astragalus can reduce the inflammatory response and exert its protective effect on the brain by reducing the content of IL-1β and MPO activity in cerebral cortex after ischemia-reperfusion.