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目的:观察辅助性17 T细胞(Th17)及其分泌的细胞因子IL-17在自身免疫性溶血性贫血(AIHA)患者中的变化并探讨其临床意义。方法:应用流式细胞术(FCM)检测治疗前与糖皮质激素治疗后AIHA患者和健康人外周血中Th17细胞的比率,同时用ELISA法检测血浆中IL-17的水平。结果:治疗前AIHA患者外周血中Th17细胞比率和血浆中IL-17的水平分别为(2.78±0.59)%和(126.4±11.6)ng/L,较健康对照[分别为(0.59±0.15)%和(52.3±4.8)ng/L]显著增高(P<0.01);治疗后,外周血中Th17细胞比率和血浆中IL-17的水平[分别为(1.05±0.28)%和(78.5±6.4)ng/L]较治疗前下降(P<0.01)。结论:AIHA患者外周血中Th17细胞的比率及其IL-17的水平均高,用糖皮质激素治疗后可下调Th17细胞的比率及IL-17的水平,有效地缓解AIHA。
Objective: To investigate the changes of Th17 and its secreting cytokine IL-17 in patients with autoimmune hemolytic anemia (AIHA) and to investigate its clinical significance. Methods: Flow cytometry (FCM) was used to detect the ratio of Th17 cells in the peripheral blood of AIHA patients and healthy people before and after treatment with glucocorticoid. Meanwhile, the level of IL-17 in plasma was detected by ELISA. Results: The levels of Th17 cells and IL-17 in plasma of AIHA patients before treatment were (2.78 ± 0.59)% and (126.4 ± 11.6) ng / L, respectively, which were significantly higher than those of healthy controls [0.59 ± 0.15% And (52.3 ± 4.8) ng / L] were significantly increased (P <0.01). After treatment, the levels of Th17 cells and IL-17 in plasma were (1.05 ± 0.28)% and (78.5 ± 6.4) ng / L] than before treatment (P <0.01). Conclusion: Th17 cells in peripheral blood of AIHA patients and the levels of IL-17 are all high. After treatment with glucocorticoid, the ratio of Th17 cells and the level of IL-17 can be decreased, which can effectively relieve AIHA.