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采用紫外分光光度法测定了不同月份忽地笑鳞茎中总生物碱含量,为确定适宜的采收时间提供实验依据。结果表明,忽地笑鳞茎中总生物碱含量随生长发育时期或月份不同存在一定差异,其中11月的最高,12月次之,1月的最低,最高值与最低值相比,约为2.6倍,差异达极显著水平(P<0.01)。精密度、稳定性实验及回收率等方法学考察表明,紫外分光光度法测定忽地笑鳞茎中总生物碱含量效果好,稳定性强,方法可靠。综合忽地笑鳞茎中总生物碱含量、中药材产量和土地利用率等因素考虑,以11月底前后采收比较适宜。
The content of total alkaloids in the bulb of Laughing Laughter and Laughter in different months was determined by UV spectrophotometry, which provided the experimental basis for determining the suitable harvesting time. The results showed that the content of total alkaloids in the bulbs of Ligusticum and Lathyrus varied with growth period or month, with the highest in November, the second in December, the lowest in January, and the highest and lowest in about 2.6 times , The difference was extremely significant (P <0.01). The study of precision, stability, recovery and other methodological studies showed that ultraviolet spectrophotometry was effective in determining the total alkaloid content in the bulbs, and its stability was high and the method was reliable. Comprehensive suddenly laugh at the total alkaloid content of the bulb, the yield of Chinese herbal medicines and land utilization and other factors to consider before and after the end of November harvest more appropriate.