论文部分内容阅读
已证实肠化与胃癌发生关系密切,但近年研究表明仅某些肠化具癌前性质。为进一步探讨胃癌发生与肠化,特别是肠化类型的关系,筛选出与胃癌发生关系最密切的肠化类型,作者对25例胃癌和19例胃良性疾病患者的胃大部切除标本进行了比较研究。从病变区至双侧大弯和小弯各取全长粘膜一块,采用光镜、粘液组化和酶组化对胃粘膜的肠化情况进行观察。以PAS/AB鉴别中性粘液和酸性粘液,以HID/AB鉴别硫酸粘液和唾酸粘液,以PB/KOH/PAS确定氧乙酰化唾酸粘液,用组化方法显示蔗糖酶和海藻酶活性。胃粘液出现杯状细胞、潘氏细胞、肠性粘液和肠标志酶均确定为肠化,并按肠化程度依次分为轻、中、重三级。
It has been confirmed that intestinal metaplasia is closely related to the occurrence of gastric cancer, but recent studies have shown that only certain intestinal metastases have precancerous properties. In order to further investigate the relationship between the occurrence of gastric cancer and intestinal metaplasia, especially the type of intestinal metastasis, the type of intestinal metastasis most closely related to the occurrence of gastric cancer was selected. The authors performed a subtotal gastrectomy specimen of 25 patients with gastric cancer and 19 patients with benign gastric disease. compare research. A piece of full-length mucous membrane was taken from the lesion area to both large and small curves, and the intestinal mucosal status was observed by light microscopy, mucus histochemistry and enzymatic histochemistry. The PAS/AB was used to identify neutral mucus and acid mucus. HID/AB was used to identify sulfated mucus and sialic acid mucus. PB/KOH/PAS was used to determine the oxyacetylated sialic acid mucus. The invertase and alginase activities were visualized by histochemical methods. Gastric mucus appearance Goblet cells, Paneth cells, intestinal mucus and intestinal marker enzymes were identified as intestinal metastases, and according to the degree of intestinal metaplasia were divided into light, medium and heavy.