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应用高脂饮食以腹腔注射小剂量链脲佐菌素的方法建立2型糖尿病大鼠模型,同时采用青心酮对糖尿病大鼠进行干预。喂养4周后检测肿瘤坏死因子-α变化。采用酶法收集胸主动脉平滑肌细胞,采用MTT法测定胸主动脉平滑肌细胞增殖程度。结果:糖尿病组大鼠血糖、血胰岛素、甘油三酯、总胆固醇、低密度脂蛋白、血清MDA以及TNF-α水平均较正常明显增高(P<0.01),血管平滑肌细胞增殖程度也明显增高(P<0.01)。给予青心酮干预治疗后,糖尿病大鼠血糖、血胰岛素、甘油三酯、总胆固醇、低密度脂蛋白无明显变化,而血清MDA、TNF-α水平较糖尿病组大鼠明显降低,血管平滑肌细胞增殖呈程度降人工(P<0.01)。结论:青心酮可以通过减轻氧化应激和炎症反应,减少血管平滑机细胞增殖程度,因此,青心酮可能成为治疗2型糖尿病大血管并发症的新途径。
The model of type 2 diabetes mellitus was established by intraperitoneal injection of small doses of streptozotocin in a high-fat diet. Tumor necrosis factor-α changes after 4 weeks of feeding. Thoracic aortic smooth muscle cells were collected by enzymatic method. The proliferation of thoracic aorta smooth muscle cells was determined by MTT assay. Results: The levels of blood glucose, blood insulin, triglyceride, total cholesterol, low density lipoprotein, serum MDA and TNF-α in diabetic rats were significantly higher than those in normal rats (P <0.01), and the proliferation of vascular smooth muscle cells P <0.01). There was no significant change in blood glucose, blood insulin, triglyceride, total cholesterol and low density lipoprotein in diabetic rats, while serum MDA and TNF-α levels were significantly lower than those in diabetic rats, and vascular smooth muscle cells Proliferation showed a decrease of artificial (P <0.01). CONCLUSION: CPP can reduce oxidative stress and inflammation and decrease the proliferation of vascular smooth muscle cells. Therefore, CPP may be a new way to treat the macrovascular complications of type 2 diabetes mellitus.