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目的:以大鼠组织匀浆及肝亚细胞为材料研究海洋新结构化合物软珊瑚胆甾烯(24-亚甲基-3β、7β、19-三羟基-5-胆甾烯)的抗氧化作用。方法:组织匀浆或肝亚细胞悬液与软珊瑚胆甾烯共浴后,以VitC+FeSO4或邻苯三酚激发脂质过氧化,以丙二醛产生,还原性谷胱甘肽耗竭,膜唾液酸耗竭、线粒体膨胀作为脂质过氧化程度指标。结果:7.6、30.6、122.2μmol·L-1软珊瑚胆甾烯可抑制VitC+FeSO4激发的丙二醛产生,阻遏肝组织匀浆还原性谷胱甘肽耗竭,线粒体膨胀,但对邻苯三酚致质膜唾液酸耗竭无显著影响。结论:软珊瑚胆甾烯具有一定的抗氧化作用,但可能并无直接清除超氧阴离子自由基(O-·2)的作用。
OBJECTIVE: To study the anti-oxidative effects of the novel marine structural compounds of soft coral cholesteene (24-methylene-3β, 7β, 19-trihydroxy-5-cholestene) using rat tissue homogenates and liver subcellular materials. . METHODS: Tissue homogenates or liver subcellular suspensions were co-bathed with soft coral cholestacene to stimulate lipid peroxidation with VitC+FeSO4 or pyrogallol to produce malondialdehyde, reduced glutathione depletion, and membrane saliva Acid depletion, mitochondrial swelling as indicators of lipid peroxidation. Results: 7.6, 30.6, 122.2 μmol·L-1 soft coral cholestene inhibited the production of MDA induced by VitC+FeSO4, and inhibited the depletion of glutathione and mitochondrial swelling in liver homogenate, but Phloroglucinol had no significant effect on plasma membrane sialic acid depletion. Conclusion: Soft coral cholesterene has some antioxidation effect, but it may not have the effect of direct elimination of superoxide anion free radical (O-·2).