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目的建立人卵巢癌裸鼠移植实体瘤模型。方法将1例人卵巢癌组织移植裸鼠,建立人卵巢癌裸鼠原代移植实体瘤模型的基础上,再将实体瘤皮下移植、实体瘤原位移植、实体瘤细胞移植裸鼠。观察裸鼠实体瘤生长和转移情况,称量其体重、子宫卵巢重、瘤重及瘤的大小,并作病理、电镜、染色体检查。结果成功地建立人卵巢癌裸鼠移植实体瘤模型,并已传至第18代,传代移植成功率100%,组织学和超微结构形态均证明该实体瘤保留了原人卵巢癌特征,有人卵巢癌染色体特征,并出现肝、脾转移。结论本研究建立人卵巢癌裸鼠移植实体瘤模型与人相似,通过18代传代和实验观察方法稳定可靠。为人卵巢癌的研究提供了理想的动物模型。
Objective To establish human ovarian cancer xenograft solid tumor model. Methods One human ovarian cancer was transplanted into nude mice and the primary transplantation tumor model of human ovarian cancer was established. Then the solid tumor was transplanted subcutaneously, the solid tumor was implanted in situ, and the solid tumor cells were transplanted in nude mice. Observe the growth and metastasis of solid tumor in nude mice, weighed its body weight, uterine ovary weight, tumor weight and tumor size, and pathology, electron microscopy, chromosome examination. Results The human ovarian cancer xenograft solid tumor model was successfully established and passed to the 18th generation. The success rate of passage transplantation was 100%. The histological and ultrastructural morphological evidences showed that the solid tumor retained the characteristics of human ovarian cancer. Ovarian cancer chromosome features, and liver and spleen metastasis. Conclusion This study established human ovarian cancer nude mice transplantation solid tumor model and human similar, through 18 generations of passage and experimental observation method is stable and reliable. The study of human ovarian cancer provides an ideal animal model.