免疫组织化学(IHC)的历史可以追溯到几十年前,但这并不意味着它已停滞不前。自动样品处理器、数字幻灯片扫描仪、图像分析软件等等,毫无疑问地给这一古老技术带来了21世纪的曙光。免疫组化技术关乎的都是空间关系。如果研究人员想要知道一段给定组织是否表达一种特定的蛋白质,他们可以使用质谱或免疫印迹。但是为了确定在组织的哪个位置发现了这种蛋白,他们必须在细胞层面对其进行可视化。这时,IHC就派上了用场。该方法利用抗体作为特异性抗原的探针, The history of immunohistochemistry (IHC) dates back decades, but that does not mean it has stalled. Automatic sample processors, digital slide scanners, image analysis software, etc. undoubtedly bring the 21st century dawn to this ancient technology. Immunohistochemistry is about spatial relationships. If researchers want to know if a given tissue expresses a particular protein, they can use mass spectrometry or immunoblotting. But in order to determine where in the tissue this protein was found, they had to visualize it at the cellular level. At this time, IHC came in handy. This method utilizes antibodies as probes for specific antigens,