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为探讨盐酸戊乙奎醚(PHC)对脂多糖(LPS)诱导的肺微血管内皮细胞通透性的影响,将Wistar大鼠肺微血管内皮细胞随机分为对照组、LPS组和LPS+PHC预处理组,测定内皮单层滤过系数(Kf)和蛋白质渗透压反射系数(σ),MTT法测定细胞存活率,免疫印迹法测定水通道蛋白-1(AQP-1)表达。与对照组比较,LPS组Kf增加、σ减少,存活率降低;PHC预处理可减少LPS组的变化,提高存活率,上调AQP-1表达。
To investigate the effect of penehyclidine hydrochloride (PHC) on lipopolysaccharide (LPS) -induced pulmonary microvascular endothelial cell permeability, pulmonary microvascular endothelial cells of Wistar rats were randomly divided into control group, LPS group and LPS + PHC pretreatment (Kf) and osmotic pressure reflectance (σ) were measured. The cell viability was measured by MTT assay and the expression of AQP-1 was detected by Western blotting. Compared with the control group, LPS group Kf increased, σ decreased, survival rate decreased; PHC pretreatment can reduce the changes in LPS group, improve survival rate, up-regulate AQP-1 expression.