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目的探讨膀胱移行细胞癌(TBCC)中Survivin表达的意义。方法应用免疫组织化学一步法、RT-PCR法检测40例BTCC和12例正常膀胱粘膜中Survivin和Survivin mRNA的表达,Western blot方法检测40例BTCC与32例非BTCC患者尿液细胞中Survivin的表达。结果免疫组织化学方法显示,Survivin在BTCC中阳性表达率为95.0%,在正常膀胱粘膜中无表达(P<0.01)。RT-PCR显示,Survivin mRNA在BTCC中阳性表达率为100.0%,在正常粘膜中无表达(P<0.01)。Western blot显示,Survivin在BTCC患者尿液细胞中阳性表达率为95.0%,在非BTCC患者中无表达(P<0.01)。3组中Survivin的表达与BTCC的分级、分期密切相关(P<0.01)。结论Survivin在BTCC中高表达,因而可作为BTCC的一个良好的诊断和预后指标。
Objective To investigate the significance of Survivin expression in bladder transitional cell carcinoma (TBCC). Methods The expressions of Survivin and Survivin mRNA in 40 BTCCs and 12 normal bladder mucosa were detected by immunohistochemistry and RT-PCR. The expressions of Survivin in 40 BTCC and 32 non-BTCC were detected by Western blot . Results Immunohistochemistry showed that the positive rate of Survivin in BTCC was 95.0%, which was not found in normal bladder mucosa (P <0.01). The positive rate of Survivin mRNA in BTCC was 100.0% by RT-PCR and no expression in normal mucosa (P <0.01). Western blot showed that the positive rate of Survivin in urinary cells was 95.0% in BTCC patients and no expression in non-BTCC patients (P <0.01). The expression of Survivin in 3 groups was closely related to the grade and stage of BTCC (P <0.01). Conclusion Survivin is highly expressed in BTCC, which may be a good diagnostic and prognostic indicator of BTCC.