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作者在本题的研究中设计了如下实验: 用脱鞘的犬钩虫感染性幼虫制成3种抗原:(1)在室温下(20℃),将幼虫置于pH 7.2的磷酸缓冲液(PBS)中;(2)幼虫放置液氮罐内24小时后,在37℃下迅速融化,加入0℃的PBS后,分别在0℃和20℃PBS中测试;(3)幼虫置于含20mM的叠氮化合物的PBS中1小时后,在室温下直接试验、另一部分幼虫用PBS洗涤3次除去叠氮物后在20 C PBS
In this study, the authors designed the following experiments: Three kinds of antigens were made from the infecting larvae of hookworm H. canis: (1) The larvae were placed in phosphate buffered saline (PBS) at pH 7.2 at room temperature (20 ° C) (2) The larvae were placed in liquid nitrogen tanks for 24 hours and then rapidly melted at 37 ° C. After adding PBS at 0 ° C, the larvae were respectively tested in PBS at 0 ° C and 20 ° C. (3) Nitrogen compound in PBS for 1 hour, the direct test at room temperature, the other part of the larvae washed three times with PBS to remove the azide in 20 C PBS