论文部分内容阅读
目的： 通过对细胞周期蛋白（ｃｙｃｌｉｎ） Ｄ１ 及其与细胞增殖和细胞凋亡在牙齿发生、发育过程中的关系研究，以探讨牙齿发育的机制。方法：选用纯系 Ｓ Ｄ 大鼠建立牙齿发育模型，采用原位末端标记法（ Ｔ Ｕ Ｎ Ｅ Ｌ 法） 、免疫组织化学染色技术，观察分析纯系 Ｓ Ｄ 大鼠牙胚发育不同阶段ｃｙｃｌｉｎ Ｄ１ 的表达及其与增殖细胞核抗原（ Ｐ Ｃ Ｎ Ａ） 和细胞凋亡的关系。结果：在牙胚发育的蕾状期、帽状期、钟状早期的细胞增殖中心处三者表达都很明显。钟状晚期及牙体组织形成后，已分化的成釉细胞、成牙本质细胞中ｃｙｃｌｉｎ Ｄ１ 表达阴性， Ｐ Ｃ Ｎ Ａ及凋亡阳性细胞也减少，但牙乳头及星网层细胞中可见二者少量阳性细胞。结论：ｃｙｃｌｉｎ Ｄ１ 的表达与 Ｐ Ｃ Ｎ Ａ和细胞凋亡有内在的相互作用关系，三者共同在牙齿发育中发挥重要的调控作用。
OBJECTIVE: To investigate the mechanism of tooth development by studying the relationship between cyclin D1 and cell proliferation and apoptosis during tooth development and development. Methods: Dental development model was established in pure SD rats. TUNEL method and immunohistochemical staining were used to observe the expression of cyclin D1 And its relationship with proliferating cell nuclear antigen (PCNA) and apoptosis. Results: The expression of all three was obvious in the budding stage, cap stage and early bell-shaped cell proliferation center of tooth germ development. After the formation of the bell-shaped and tooth formation, the differentiated ameloblasts, odontoblast expression of cyclin D1 negative, P C N A and apoptosis-positive cells also decreased, but the teeth papillary and satellite layer cells can be seen in two A small amount of positive cells. CONCLUSION: The expression of cyclin D1 has an intrinsic interaction with PCNA and apoptosis. All three play an important role in the regulation of tooth development.