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将白菜绒毡层特异启动子BcA9驱动下的核糖核酸酶Barnase基因,通过农杆菌介导的下胚轴转化导入甘蓝材料‘TF’,获得具有除草剂Basta抗性的甘蓝转基因植株.经PCR鉴定,BcA9-Barnase融合基因已经整合到甘蓝基因组中.细胞学观察显示,转基因甘蓝植株的花药因绒毡层细胞提前降解,花粉母细胞退化,成熟花药中无正常花粉粒产生.相对非转基因对照,转基因甘蓝植株花器官变小,花瓣褶皱多,柱头弯曲,雄蕊瘦小等现象.另外,转基因植株在温度低于22℃时出现明显的死蕾现象,当温度高于25℃时,死蕾现象得到缓解.田间结实情况调查显示,转基因甘蓝植株自交不能结实,与野生型杂交可结实但种荚变短.
The Barnase gene driven by the BcA9 promoter of cabbage tapetum was introduced into Brassica oleracea ’TF’ by Agrobacterium tumefaciens-mediated hypocotyl transformation to obtain the B. tabaci transgenic plants with Basta resistance. , The BcA9-Barnase fusion gene has been integrated into Brassica oleracea L. Cytological observations showed that the anther of transgenic cabbage plants degenerated prematurely, pollen mother cells degenerated, and no normal pollen grains were produced in mature anthers.Compared with non-transgenic control, In addition, the transgenic plants showed obvious dead buds when the temperature was lower than 22 ℃. When the temperature was higher than 25 ℃, the dead bud was obtained Alleviation field field conditions survey showed that the selfing of transgenic cabbage plants can not be strong, and the wild-type hybrid can be strong but the seed pods shorter.