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目的:建立抗感合剂含量测定方法。方法:采用液相色谱法测定黄芩苷含量。以shim-pakclc-ODS(4.6×150mm)为色谱柱,以甲醇0.025mol/L磷酸(30:70)为流动相,检测波长为278nm。结果:黄芩苷的对照品进样量在0.2～2.0ug范围内线性关系良好。方法的回收率为98.3%(n=5)。RSD为1.7%。结论:方法快速、准确,方法的稳定性、重复性良好。可用于抗感合剂的质量控制。
Objective: To establish a method for determining the content of anti-infectives. Methods: The content of baicalin was determined by liquid chromatography. A shim-pakclc-ODS (4.6×150 mm) was used as the column and methanol 0.025 mol/L phosphoric acid (30:70) was used as the mobile phase. The detection wavelength was 278 nm. RESULTS: The linearity of the baicalin in the range of 0.2-2.0 μg was very good. The recovery of the method was 98.3% (n=5). RSD is 1.7%. Conclusion: The method is rapid and accurate. The stability and repeatability of the method are good. Can be used to control the quality of anti-allergy agents.