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Objective: To investigate the relationship between urokinase type plasminogen activator(uPA) activityand the malignant biological behavior of human brain tumors. Methods: The amounts of uPA were measured byNorthern blot hybridization and immunohistochemical methods in 43 cases of brain gliomas and 5 cases of normalbrain tissues,and their relation with the clinical indexes was comprehensively analyzed. Results: All tissues expressed 2. 5 kb transcripts of uPA mRNA. The uPA mRNA levels were significantly higher in high-grade gliomasthan in low-grade gliomas and normal brain tissues (P<0.01),but no remarkable difference was observed betweenlow-grade gliomas and normal brain tissues (P>0.05). Levels of uPA mRNA expression were significantly higherin tumor tissues with recurrences in 18 months postoperatively and a survival period of less than 3 years than intheir counterparts withollt postoperative recurrence in 18 months and with a survival period of more than 3 years(P<0. 01 ). The uPA mRNA was also expressed in tumor cells near necrotic areas. The uPA mRNA expressionwas considerably correlated with the microvessel quantity (MVQ) in gliomas (r= 0. 56, P<0.01),but had no significant correlation with the sex or age of patients and their tumor sizes. The uPA protein was mainly distributed intumor cells and endothelial cells of glioblastomas and anaplastic astrocytomas,whereas it was almost undetectablein low-grade gliomas. Conclusion: The expression of uPA gene is probably associated with the gliomas malignity,playing an irnportant role in predicting the recurrences and invasive behaviors of higher grade gliomas.
Methods: The amounts of uPA were measured by Norther blot hybridization and immunohistochemical methods in 43 cases of brain gliomas and 5 cases of normalbrain tissues Results: All tissues expressed 2.5 kb transcripts of uPA mRNA. The uPA mRNA levels were significantly higher in high-grade gliomasthan in low-grade gliomas and normal brain tissues (P < 0.01), but no remarkable difference was observed between low-grade gliomas and normal brain tissues (P> 0.05). Levels of uPA mRNA expression were significantly higher in tumor tissues with recurrences in 18 months postoperatively and a survival period of less than 3 years than intheir counterparts withollt postoperative recurrence in 18 months and with a survival period of more than 3 years (P <0. 01). The uPA m RNA was also expressed in tumor cells near necrotic areas. The uPA mRNA expression was significantly correlated with the microvessel quantity (MVQ) in gliomas (r = 0.56, P <0.01), but had no significant correlation with the sex or age of patients and its tumor sizes. The uPA protein was mainly distributed in tumor cells and endothelial cells of glioblastomas and anaplastic astrocytomas, it it almost undetectablein low-grade gliomas. Conclusion: The expression of uPA gene is probably associated with the gliomas malignity, playing an irnportant role in predicting the recurrences and invasive behaviors of higher grade gliomas.