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目的 观察转化生长因子β（ｔｒａｎｓｆｏｒｍｉｎｇ ｇｒｏｗ ｔｈ ｆａｃｔｏｒ－ｂｅｔａ，ＴＧＦ－β）和干扰素γ（ｉｎｔｅｒｆｅｒｏｎ－ｇａｍ ｍ ａ，ＩＦＮ－γ）对人视网膜色素上皮细胞（ｒｅｔｉｎａｌｐｉｇｍ ｅｎｔｅｐｉｔｈｅｌｉａｌｃｅｌｌｓ，ＲＰＥ）胶原合成的影响。 方法 用３Ｈ－脯氨酸掺入、免疫细胞化学及分子杂交技术观察ＴＧＦ－β和ＩＦＮ－γ分别及联合作用下培养的人ＲＰＥ胶原合成活性。 结果 ＴＧＦ－β在１０ ｎｇ／ｍｌ浓度使ＲＰＥ对３Ｈ－脯氨酸的摄取增加１３０．８７％ ，并增强Ⅳ、Ⅰ和Ⅲ型胶原荧光染色和ｍ ＲＮＡ表达。ＩＦＮ－γ在１０ ０００ Ｕ／ｍ ｌ浓度抑制５４．７２％ 的脯氨酸摄取，并减弱Ⅳ型胶原染色和３种胶原的ｍ ＲＮＡ表达。 结论 ＴＧＦ－β和ＩＦＮ－γ对ＲＰＥ的胶原合成分别具有正向及负向调控作用，ＩＦＮ－γ可能用于抑制ＲＰＥ胶原合成。
Objective To observe the effects of transforming growth factor-β (TGF-β) and interferon-γ (IFN-γ) on the collagen synthesis of human retinal pigment epithelial cells (RPE). Methods 3H-Proline incorporation, immunocytochemistry and molecular hybridization were used to observe the collagen synthesis activity of human RPE cultured in vitro and in combination with TGF-β and IFN-γ respectively. Results TGF-β increased the uptake of 3H-proline by 130.87% in 10 ng / ml RPE, and enhanced the expression of collagen type IV, Ⅰ and Ⅲ and m RNA. IFN-γ inhibited proline uptake by 54.72% at a concentration of 10,000 U / ml and attenuated type IV collagen staining and mRNA expression of three types of collagen. Conclusion TGF-β and IFN-γ have positive and negative regulatory effects on collagen synthesis of RPE, IFN-γ may be used to inhibit RPE collagen synthesis.