论文部分内容阅读
目的检测食管鳞癌组织中Mel-18 mRNA的表达,探讨其在食管鳞癌发生发展中的作用及其临床意义。方法 53例食管鳞癌手术标本及配对癌旁正常食管组织标本(距肿瘤5 cm以上)选自山东大学附属千佛山医院胸外科的手术患者,采用逆转录多聚酶链反应(RT-PCR)方法检测两组标本中Mel-18的表达,并分析其在肿瘤中的表达情况与各临床病理因素(性别,肿瘤部位、大小、分化程度、浸润深度、临床分期、淋巴结转移)的关系。结果食管鳞癌组织中的Mel-18 mRNA表达水平显著低于癌旁正常食管组织(P<0.01)。Mel-18 mRNA在不同性别及肿瘤部位、大小、分化程度和浸润深度间的表达均无明显差异(P>0.05)。Mel-18基因在临床分期Ⅰ、Ⅱ、Ⅲ、Ⅳ期患者中的表达依次降低(P<0.05),在淋巴结转移组和无淋巴结转移组的表达差异有统计学意义(P<0.05)。结论 Mel-18基因在食管鳞癌的发生发展中可能起抑制作用,可望成为食管鳞癌明确诊断及判断预后的新指标之一。
Objective To detect the expression of Mel-18 mRNA in esophageal squamous cell carcinoma and explore its role in the development of esophageal squamous cell carcinoma and its clinical significance. Methods 53 specimens of esophageal squamous cell carcinoma and paired paracancerous normal esophageal specimens (above 5 cm from the tumor) were selected from thoracic surgery patients at Qianfoshan Hospital Affiliated to Shandong University, and detected by reverse transcription-polymerase chain reaction (RT-PCR). The expression of Mel-18 in the two groups of specimens was analyzed, and the relationship between their expression in the tumor and various clinical pathological factors (sex, tumor location, size, differentiation, depth of invasion, clinical stage, lymph node metastasis) was analyzed. Results The expression of Mel-18 mRNA in esophageal squamous cell carcinoma tissues was significantly lower than that in normal tissues (P<0.01). There was no significant difference in the expression of Mel-18 mRNA among different sexes and tumor sites, sizes, differentiation and infiltration depth (P>0.05). The expression of Mel-18 gene in patients with clinical stages I, II, III, and IV decreased in turn (P<0.05). There was a significant difference in the expression of Mel-18 gene in lymph node metastasis group and no lymph node metastasis group (P<0.05). Conclusion Mel-18 gene may play an inhibitory role in the occurrence and development of esophageal squamous cell carcinoma. It may be one of the new indicators for the definite diagnosis and prognosis of esophageal squamous cell carcinoma.