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目的探讨共刺激分子在Graves病(GD)的甲状腺组织中的表达及其免疫病理意义。方法运用细胞培养、流式细胞术和RT-PCR技术,检测10例GD组织和10例非毒性甲状腺肿(NTG)组织中共刺激分子基因的表达水平,原代培养的甲状腺滤泡细胞(TFCs)共刺激分子的表达及细胞因子对其表面共刺激分子表达的调节作用。结果GD组织一系列共刺激分子基因的表达明显高于NTG组,其中共刺激分子CD40、CD40L、OX40L和ICOS基因表达尤为显著;炎症类细胞因子可上调原代培养的TFCs表达共刺激分子CD40和GL50。结论共刺激分子在GD组织异常表达,提示CD40-CD40L、ICOS-GI50及OX40-OX40L信号在GD的局部免疫病理过程中起重要作用。
Objective To investigate the expression of costimulatory molecules in thyroid tissue of Graves disease (GD) and its immunopathological significance. Methods The expression of co-stimulatory molecules in 10 cases of GD and 10 cases of non-toxic goiter (NTG) were detected by cell culture, flow cytometry and RT-PCR. Primary cultured thyroid follicular cells (TFCs) Costimulatory molecules and regulation of cytokines on the expression of costimulatory molecules on their surface. Results The expression of co-stimulatory molecules in GD tissues was significantly higher than that in NTG group. The co-stimulatory molecules such as CD40, CD40L, OX40L and ICOS were highly expressed. Inflammatory cytokines could up-regulate the co-stimulatory molecules CD40 GL50. Conclusions Co-stimulated molecules are abnormally expressed in GD tissues, suggesting that CD40-CD40L, ICOS-GI50 and OX40-OX40L signals play an important role in the local immunopathological process of GD.