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目的观察以健脾补肾-涤痰祛瘀法为指导的敦煌石室大宝胶囊(DHDB)对衰老模型大鼠血清白细胞介素(IL)-4、IL-10含量和海马区热休克蛋白(HSP)86基因和蛋白表达的影响。方法受试动物按随机数字表法分为空白组(n=10)和模型组(n=50)。采用D-半乳糖(0.125 g/kg)皮下注射建立衰老模型并筛选符合衰老模型的大鼠,按随机数字表法分为模型组、DHDB高、中、低剂量组(0.8、0.4、0.2 g/kg)和阳性对照组(0.4 g/kg吡拉西坦),经药物干预后分别测定各组大鼠血清IL-4、IL-10含量和海马区HSP86基因和蛋白表达水平。结果与空白组比较,模型组大鼠学习能力下降,血清IL-4含量显著降低、IL-10含量显著增高,海马区HSP86基因和蛋白表达水平显著降低(均P<0.05);DHDB各剂量组大鼠学习能力提高,血清IL-4含量显著增高而IL-10含量显著降低,海马区HSP86基因和蛋白表达水平显著增高,且以高剂量DHDB作用为优(P<0.05)。结论 DHDB具有提高衰老大鼠学习记忆能力,调节血清IL-4、IL-10含量正常分泌及促进海马区HSP86基因和蛋白表达的作用。
Objective To observe the effect of Dunhuang Shishi Dabao Capsule (DHDB) guided by spleen and nourishing kidney - dispersing phlegm and removing blood stasis on the levels of serum interleukin (IL) -4 and IL-10 and the level of heat shock protein (HSP) in hippocampus of aged rats, 86 gene and protein expression. Methods The animals were divided into blank group (n = 10) and model group (n = 50) by random number table. The model of aging was established by subcutaneous injection of D-galactose (0.125 g / kg) and the aging model was screened. The rats in model group were divided into model group by random number table. The high, middle and low dose DHDB group (0.8, 0.4, 0.2 g / kg) and positive control group (0.4 g / kg piracetam). After intervention, the levels of IL-4 and IL-10 in serum and the expression of HSP86 gene and protein in hippocampus were determined. Results Compared with the blank group, the learning ability of the model group decreased, serum IL-4 level was significantly decreased, IL-10 content was significantly increased, hippocampus HSP86 gene and protein expression levels were significantly lower (all P <0.05) The learning ability of rats was improved, serum IL-4 content was significantly increased and IL-10 content was significantly decreased. The expression of HSP86 gene and protein in hippocampus was significantly increased, and high-dose DHDB was the best (P <0.05). Conclusion DHDB can improve learning and memory of aging rats, regulate the secretion of IL-4 and IL-10 and promote the expression of HSP86 in hippocampus.