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Objective Constructing replication defect type virus vector of yeast cytosine deaminase gene expression guided by hTERT core promoter modified by OSE2 tandem replicator.Methods Multimer of OSE2 was designed and cloned into the upstream site of hTERT core promoter,then confirmed by sequencing.Shuttle plasmids were constructed using modified hTERT promoter and Fcy1 and cotransfected HEK 293 cells.Recombination yields replication defect type adenovirus.Result HEK 293 cells cotransfected by shuttle plasmid and assisted plasmid appeared typical cytopathic effect 7～ 10 days later.122bp objective fragment was confirmed by PCR evaluation.Conclusion We successfully constructed the replication defective adenovirus of Fcy1 gene expression guided by hTERT core promoter modified by 3 or 6 copys OSE2.