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目的 :克隆表达人成纤维细胞生长因子受体。方法 :采用RT PCR法。结果 :在钓取人cDNA的过程中 ,意外地钓取到 2个与FGFR1细胞外段同源的cDNA片段 ,经克隆和DNA序列分析发现它们是FGFR1细胞外源的cDNA片段。结论 :虽然对这 2个cDNA片段的功能尚不明确 ,但这种抓住新现象并进行探究的意识对科研很重要
OBJECTIVE: To clone and express human fibroblast growth factor receptor. Methods: RT PCR method was used. Results: Two cDNA fragments homologous to the extracellular domain of FGFR1 were inadvertently captured during the process of human cDNA retrieval. They were found to be exogenous cDNA fragments of FGFR1 by cloning and DNA sequence analysis. Conclusion: Although the function of these two cDNA fragments is unclear, this awareness of new phenomena and explorations is important for scientific research