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目的:研究荔枝黄酮提取分离工艺及其产物黄酮组分构成。方法:采用紫外吸收芦丁标准曲线法测定荔枝黄酮含量,比较乙醇水溶液和酸化乙醇水溶液提取荔枝黄酮效果,优化大孔树脂分离荔枝黄酮粗提物,采用HPLC图谱法分析荔枝黄酮提取分离产物的黄酮组分。结果:采用紫外吸收芦丁标准曲线法检测荔枝黄酮含量方法简便、有效;70%乙醇水溶液提取荔枝黄酮较其酸化溶液有更好的提取效果,且粗提物黄酮组分更简单,该法提取荔枝皮黄酮粗提物得率达到(92.5±2.0)%,纯度(28.7±1.8)%;在比较的6种大孔树脂中,AB-8分离荔枝黄酮效果最好,以80%乙醇水溶液为洗脱剂分离荔枝黄酮,得率为(86.5±2.1)%,纯度达到(68.6±1.5)%。结论:采用70%乙醇水溶液提取结合大孔树脂AB-8分离荔枝黄酮工艺简单而有效。
Objective: To study the extraction and separation of flavonoids from litchi and the composition of its flavonoids. Methods: The content of flavonoids in litchi was determined by UV-absorbing rutin standard curve method. The effects of ethanol solution and acidified ethanol solution on the extraction of flavonoids from litchi were compared. The macroporous resin was used to separate the crude flavonoids from litchi. The HPLC analysis was used to analyze the flavonoids of lychee flavonoids. Components. Results: The method of detecting rutin content of lychees by UV-absorbing rutin standard curve method was simple and effective. The extraction of flavonoids from litchi with 70% aqueous ethanol solution had better extraction effect than its acidified solution, and the flavonoid components of crude extract were simpler. The yield of crude flavonoids from Litchi flavonoids reached (92.5±2.0)% and the purity was (28.7±1.8)%. Of the six macroporous resins, AB-8 had the best effect of separating Litchi flavonoids. The 80% ethanol solution was used as the extract. The eluent was used to isolate flavonoids from litchi. The yield was (86.5±2.1)% and the purity was (68.6±1.5)%. Conclusion: The extraction of flavonoids from litchi by using 70% ethanol aqueous solution combined with macroporous resin AB-8 is simple and effective.