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目的采用野外小规模实验和室内分析相结合的方法验证3株真菌YNAS04、YNAS06和YNAS08诱导白木香产生沉香的可行性。方法采用真菌诱导法诱导白木香产生沉香,利用气相-质谱联用技术(GC-MS)对诱导结香材料挥发油成分中的化合物进行检测,通过比对诱导材料高频菌与原接入菌的形态及核糖体基因内部转录间隔区序列(internal transcribed spacer,ITS)一致性来验证接入菌的有效性。结果 3株真菌定植于白木香6个月,YNAS04、YNAS06和YNAS08诱导产生材料的重量分别为(1.786±0.473),(2.964±0.492)和(3.615±0.591)g,高于空白对照(1.325±0.407)g和阴性对照(1.462±0.417)g。GC-MS检测到3株活性真菌诱导材料含有代表沉香品质的主要化学成分倍半萜类化合物和色原酮类似物。菌种验证结果表明,3株活性真菌在诱导材料中为优势菌,分别占分离菌种的90.0%,97.5%和91.25%。结论利用3株活性真菌诱导白木香产生沉香可以应用于实际生产,是可持续、高效生产沉香的良好方法。
OBJECTIVE To verify the feasibility of inducing alopecia incense in white wood with three strains of fungi YNAS04, YNAS06 and YNAS08 by field experiments and laboratory analysis. Methods Incense-inducing method was used to induce alopecuroylin and the compounds in volatile oil components of GC-MS were detected by gas chromatography-mass spectrometry (GC-MS). By comparing the inducing materials with the original bacteria Morphology and the internal transcribed spacer (ITS) sequence of ribosomal genes to verify the effectiveness of access bacteria. Results The weight of materials induced by YNAS04, YNAS06 and YNAS08 were (1.786 ± 0.473), (2.964 ± 0.492) and (3.615 ± 0.591) g, respectively, higher than that of the blank control (1.325 ± 0.407) g and the negative control (1.462 ± 0.417) g. GC-MS detected three active fungi-inducing materials containing sesquiterpenoids and chromogen analogues, which were the main chemical constituents of Almonds. The results of the strain validation showed that the three active fungi were dominant bacteria in the inducing materials, accounting for 90.0%, 97.5% and 91.25% of the isolates, respectively. Conclusion The results showed that the use of three active fungi to induce the alopeculysis of Baimuxiang could be applied to practical production and it is a good method to produce aloe vera continually and efficiently.