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矮牡丹(Paeonia suffruticosa subsp.spontanea)是中国特有的濒危物种.从山西省的稷山、永济和陕西省的铜川、华山4个居群32个个体中提取了基因组DNA,并采用PAPD标记揭示了矮牡丹的遗传多样性.用16个PAPD引物共扩增出173个可重复的片段,其中有117个多态性片段,占总数的67.6％.铜川居群与其他居群分化较大.AMOVA分析表明,矮牡丹各居群间的变异为52％,各居群内总的遗传变异为48％.就单个居群而言,除永济居群内个体间差异较高(60％)外,其他3个居群内各自的遗传变异为33％～37％.根据该物种遗传结构和基础生物学研究提出了保护策略的建议.
Paeonia suffruticosa subsp.spontanea is a endemic endangered species in China.The genomic DNA was extracted from 32 individuals in 4 populations of Jishan, Yongji in Shanxi Province and Tongchuan and Huashan in Shaanxi Province and revealed by PAPD markers A total of 173 repeatable fragments were amplified by 16 PAPD primers, of which 117 were polymorphic, accounting for 67.6% of the total. The Tongchuan population differed greatly from other populations. AMOVA analysis showed that the variation among dwarf populations was 52%, and the total genetic variation within each population was 48% .For the single population, except the Yongji population, there was a significant difference (60%) , And the genetic variability in each of the other three populations was 33% -37% .According to the genetic structure and basic biology of the species, the suggestion of protection strategy was proposed.