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目的:研究槲皮素抑制人胃癌MGC803细胞增殖和诱导凋亡的作用。方法:采用MTT比色法检测不同终浓度的槲皮素对MGC803细胞增殖的影响及其细胞毒活性。应用TUNEL(TdTmediateddUTPnickendlabeling)染色法检测槲皮素诱导MGC803细胞凋亡的作用。用免疫组化法测定P16、P53和Cmyc的表达率。结果:在40～100μmol/L范围内,槲皮素可显著抑制MGC803细胞增殖(P<0.01),且呈剂量、时间依赖性。TUNEL染色显示,槲皮素诱导48h后,MGC803细胞的凋亡率明显高于对照组(P<0.01)。免疫组化检测表明,用药组P53及Cmyc蛋白的表达下降,P16蛋白的表达率增强,与对照组相比较均有显著性差异(P<0.01)。结论:槲皮素可抑制人胃癌MGC803细胞增殖,并能诱导其凋亡,其作用机制可能与下调P53及Cmyc蛋白的表达、上调P16蛋白的表达有关。
Objective: To study the effect of quercetin on the proliferation and apoptosis of human gastric cancer cell line MGC803. Methods: MTT assay was used to determine the effect of different concentrations of quercetin on the proliferation of MGC803 cells and its cytotoxicity. The effect of quercetin on the apoptosis of MGC803 cells was detected by TUNEL assay (TdTmediateddUTPnickendlabeling). Immunohistochemistry was used to determine the expression of P16, P53 and Cmyc. Results: Quercetin significantly inhibited the proliferation of MGC803 cells in the range of 40 ~ 100μmol / L (P <0.01) in a dose-and time-dependent manner. TUNEL staining showed that the apoptosis rate of MGC803 cells was significantly higher than that of the control group (P <0.01) 48h after quercetin induction. The results of immunohistochemistry showed that the expressions of P53 and Cmyc protein decreased and the expression of P16 protein increased in the treatment group compared with the control group (P <0.01). CONCLUSION: Quercetin can inhibit the proliferation and induce the apoptosis of human gastric cancer MGC803 cells. Quercetin may be involved in down-regulation of P53 and Cmyc expression and up-regulation of P16 protein expression.