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目的探讨精液经密度梯度离心时经水平转子和角转子处理后对精液质量及其对人工授精结局的影响。方法回顾性分析352个人工授精周期,根据梯度离心时转子的不同,分成水平转子组和角转子组两组,精液经密度梯度离心法处理,形态学分析严格按照WHO(World Health Organization)人类精液检验与处理实验手册第五版标准,比较处理后精液质量及两组对妊娠结局的影响。结果(1)精液质量比较:水平转子组前向运动精子为(89.2±8.5)%、精子浓度为(47.8±14.3)×10~6/mL、前向运动精子总数为(13.6±6.3)×10~6;角转子组前向运动精子为(90.1±8.8)%、精子浓度为(43.4±15.1)×10~6/mL、前向运动精子总数为(12.9±6.1)×10~6,无统计学差异(P>0.05)。水平转子组和角转子组正常精子比率分别为(18.5±8.2)%和(14.2±5.7)%,两者存在显著性差异(P<0.05);(2)妊娠结局比较:水平转子组的临床妊娠率为16.5%(30/182)、流产率为3.3%(1/30)、活产率为96.7%(29/30);角转子组生化妊娠率为临床妊娠率为12.4%(21/170)、流产率为9.5%(2/21)、活产率为90.5%(19/21),无统计学差异(P>0.05),生化妊娠率:两组分别为0%(0/30),19.2%(5/26),有统计学意义(P<0.05)。结论密度梯度离心应用水平转子可以降低对处理后精液的精子形态的影响,减少生化妊娠率。
Objective To investigate the effects of horizontal rotors and angle rotors on the quality of semen and its effect on the artificial insemination after density gradient centrifugation. Methods 352 artificial insemination cycles were retrospectively analyzed. According to different rotors in gradient centrifugation, the animals were divided into two groups: horizontal rotator group and angle rotator group. Semen was processed by density gradient centrifugation. Morphological analysis was performed according to WHO (World Health Organization) The fifth edition of the Manual of Tests and Treatments was compared with the quality of the treated semen and the effect of the two groups on the pregnancy outcome. Results (1) Comparison of semen quality: The level of forward spermatozoa was (89.2 ± 8.5)%, the sperm concentration was (47.8 ± 14.3) × 10 ~ 6 / mL and the total number of forward spermatozoa was (13.6 ± 6.3) × The number of spermatozoa in the cornea was (90.1 ± 8.8)%, the sperm concentration was (43.4 ± 15.1) × 10 ~ 6 / mL, the total number of the forward spermatozoa was (12.9 ± 6.1) × 10 ~ No statistical difference (P> 0.05). The normal spermatozoa of the horizontal rotator group and the angle rotator group were (18.5 ± 8.2)% and (14.2 ± 5.7)%, respectively, with significant difference (P <0.05). (2) Comparison of pregnancy outcome: The pregnancy rate was 16.5% (30/182), the abortion rate was 3.3% (1/30) and the live birth rate was 96.7% (29/30). The rate of biochemical pregnancy in the rotatorion group was 12.4% (21 / 170). The rate of abortion was 9.5% (2/21) and the live birth rate was 90.5% (19/21), with no significant difference (P> 0.05). The rate of biochemical pregnancy was 0% (0/30 ), 19.2% (5/26), respectively, with statistical significance (P <0.05). Conclusions The application of horizontal rotor by density gradient centrifugation can reduce the influence of sperm morphology of treated semen and reduce the rate of biochemical pregnancy.