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目的:研究艾纳香二氢黄酮对脂质过氧化致损伤的恒河猴原代培养肝细胞及肝亚细胞的保护作用。方法:艾纳香二氢黄酮与原代培养肝细胞共育后,以CCl4或FeSO4半胱氨酸致细胞损伤,以脂质过氧化产物丙二醛产生量及转氨酶的漏出量作为损伤程度指标。亚细胞结构与艾纳香二氢黄酮共育后,以FeSO4半胱氨酸致脂质过氧化,以丙二醛产生量衡量过氧化损伤程度。结果:10及100μmol·L1的艾纳香二氢黄酮可降低被损伤的肝细胞中的丙二醛产生,降低转氨酶漏出,抑制肝亚细胞结构中丙二醛生成。结论:艾纳香二氢黄酮具有抑制脂质过氧化作用及肝细胞、亚细胞结构保护作用
Aims: To study the protective effect of anthraquinone on the primary culture of hepatocytes and hepatocytes in rhesus monkey induced by lipid peroxidation. Methods: Anergen flavonoids and primary cultured hepatocytes co-cultured with CCl4 or FeSO4 cysteine induced cell injury to the lipid peroxidation product malondialdehyde and transaminase leakage as the degree of injury index. After co-culture of sub-cellular structure with anthocyanin flavonoids, lipid peroxidation was induced by FeSO4-cysteine, and the extent of peroxidative damage was measured by the amount of malondialdehyde produced. Results: 100 and 100μmol·L -1 Aitane flavonoids can reduce the malondialdehyde in injured liver cells, reduce transaminase leakage, inhibit the formation of malondialdehyde in the liver subcellular structure. Conclusions: Arteriol Flavonoids can inhibit lipid peroxidation and hepatocyte and subcellular structure protection