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目的探讨肝再生中肝脏胞外基质成分与卵圆细胞的相互关系及作用。方法采用免疫组化及免疫荧光双标的方法动态观察大鼠卵圆细胞增殖模型中肝脏胞外基质成分(层粘连蛋白和纤维连接蛋白)的定位及与卵圆细胞的关系。结果肝部分切除后第2天,卵圆细胞开始向门静脉周围区域增殖。层粘连蛋白主要出现在门静脉周围的肝窦状隙内,纤维连接蛋白在整个肝小叶内表达显著增加。术后第4~9天,卵圆细胞进一步向肝实质内增殖,门静脉周围纤维连接蛋白表达增加,中央静脉周围表达减少。层粘连蛋白及纤维连接蛋白与卵圆细胞关系紧密。术后第12~15天,随着卵圆细胞分化为小肝细胞结节,大多数层粘连蛋白及纤维连接蛋白位于结节周边,少数出现在结节内。第18天以后,正常的肝小叶结构开始恢复。结论卵圆细胞与肝脏胞外基质成分存在紧密联系;局部肝脏微环境可能通过细胞与基质之间的相互作用在卵圆细胞介导的肝再生中发挥重要的调控作用。
Objective To investigate the relationship between liver extracellular matrix components and oval cells during liver regeneration. Methods Immunohistochemistry and double immunofluorescence staining were used to dynamically observe the localization of extracellular matrix components (laminin and fibronectin) and their relationship with oval cells in rat oval cell proliferation model. Results On the second day after partial hepatectomy, oval cells began to proliferate into the peri-portal area. Laminin mainly appears in the hepatic sinusoids around the portal vein, and fibronectin is significantly increased throughout the hepatic lobule. From day 4 to day 9, the oval cells further proliferated into the parenchyma of the liver, the expression of fibronectin around the portal vein was increased, and the expression around the central vein was decreased. Laminin and fibronectin are closely related to oval cells. From the 12th to the 15th day after operation, with the differentiation of oval cells into nodules of small hepatocytes, most of the laminin and fibronectin are located around the nodules and a few appear in the nodules. After day 18, normal hepatic lobule structures begin to recover. Conclusion Oval cells are closely related to extrahepatic matrix components of liver. Local liver microenvironment may play an important regulatory role in oval cell-mediated liver regeneration through cell-matrix interactions.