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营养琼脂中加入TTC指示剂倒平板,对医院内物体表面细菌总数测定采取涂抹法培养,具有操作简单,受影响少,计数菌落清晰,检出率高等特点。1 方法:将营养琼脂溶化待冷切约45℃时,每100ml营养琼脂中加入0.15％TTC指示剂1ml(最终浓度为0.0015％)充分混匀后,倾注灭菌平皿,凝固后备用。 试验时用1ml无菌吸管吸取标本液0.2ml,注入制备好的TTC琼脂平板中心部,然后用无菌棒涂抹均匀,37℃18h后计数菌落并与琼脂平板倾注法作对照。2 结果2.1 TTC最佳浓度的选择:将不同浓度的TTC溶液配制系列不同含量的琼脂平板,对某一份标本作细菌
Nutritional agar by adding TTC indicator inverted plate, the total number of bacteria on the surface of the hospital objects to smear smear culture method has the advantages of simple operation, less affected, counting colonies clear, high detection rate. 1 Method: Dissolve nutrient agar to be cold-cut at about 45 ° C, add 0.15% TTC indicator 1ml (final concentration of 0.0015%) per 100ml nutrient agar, mix thoroughly, pour sterile plate, and set aside for use. 0.2 ml of the sample solution was aspirated with 1 ml sterile pipette into the center of the prepared TTC agar plate, and then evenly coated with a sterile rod. The colonies were counted after 18 hours at 37 ° C. and compared with the agar plate pour method. 2 Results 2.1 The best choice of TTC concentration: different concentrations of TTC solution preparation series of different levels of agar plate, a specimen of bacteria