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目的建立温肾缩尿胶囊中原儿茶酸的含量测定方法。方法色谱柱:ODS Hypersil(4.6×250mm,5μm);流动相:甲醇-水-冰醋酸(19:80:1),流速:1ml/min,检测波长:260nm,柱温:25℃。结果原儿茶酸浓度在2.228～55.70μg/ml范围内有良好线性关系(r=0.9997),平均回收率为99.73%,RSD为1.63%(n=9)。结论本测定方法专属性强,稳定可靠、简便可行、重现性好,可用于温肾缩尿胶囊的质量控制标准。
Objective To establish a method for the determination of protocatechuic acid in Wenshen Shuyu capsule. Methods Column: ODS Hypersil (4.6 × 250 mm, 5 μm); mobile phase: methanol-water-glacial acetic acid (19:80: 1); flow rate: 1 ml / min; detection wavelength: 260 nm; Results There was a good linear relationship between the concentration of protocatechuic acid and 2.228 ~ 55.70μg / ml (r = 0.9997). The average recovery was 99.73% and the RSD was 1.63% (n = 9). Conclusion The method is highly specific, stable and reliable, simple and feasible, reproducible, and can be used for the quality control standard of Wenshen Yanjiao capsule.