论文部分内容阅读
目的 观察异丙基肾上腺素 (isoproterenol,Iso)导致的心肌肥大大鼠的心肌细胞牛磺酸 (taurine ,TAU)跨膜转运功能的改变。方法 Iso诱导大鼠心肌肥厚 ;氨基酸分析仪测牛磺酸含量 ;用3H标记的牛磺酸测定心肌牛磺酸转运及牛磺酸与心肌浆膜结合功能。结果 Iso组动物较对照组的血浆和组织牛磺酸含量分别升高 49% (P <0 0 1)和降低35 % (P <0 0 1) ,心肌3H 牛磺酸摄入减少。 5min时释放量与总摄入百分比高于对照组 6 0 % (P <0 0 1) ,Iso组释放量增加。Iso心肌浆膜3H 牛磺酸结合量较对照组低 ,最大结合速率 (Bmax)较对照组低 45 % (P <0 0 5 ) ,治疗组较Iso组Bmax值增加 116 % (P <0 0 1) ,Kd值无明显差异。结论 Iso诱导的心肌损伤存在牛磺酸转运障碍 ,给予外源性牛磺酸可以有效防治其心肌牛磺酸转运障碍
Objective To investigate the transmembrane transport function of taurine (TAU) in cardiomyocytes induced by isoproterenol (Iso) in rats. Methods Isoinduced cardiac hypertrophy was induced in rats. Amino acid analyzer was used to measure the content of taurine. 3H-labeled taurine was used to determine taurine transport and taurine-to-myocardium serosal binding. Results Compared with the control group, the contents of taurine in plasma and tissue of Iso group increased by 49% (P <0.01) and 35% (P <0.01), respectively. The myocardial 3H taurine intake decreased. 5min release and total intake percentage higher than the control group 60% (P <0 0 1), Iso group release increased. The binding capacity of 3H-taurine to Iso was lower than that of the control group (P <0 0), and the maximum binding rate (Bmax) was 45% lower than that of the control group 1), Kd value no significant difference. Conclusion Iso-induced myocardial damage has taurine transport impairment, and administration of exogenous taurine can effectively prevent its cardiac taurine transport disorder