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目的探讨消朦灵对糖尿病大鼠的作用及其机制。方法雄性SD大鼠30只,10只未造模作为正常对照组;将造模后成功的20只大鼠随机分为2组:治疗组给予消朦灵方灌胃,模型对照组给予等量温开水灌胃,观察消朦灵方对大鼠全身状态、体重、血糖、SOD、MDA以及视网膜结构的影响。结果与正常对照组比较,模型对照组精神倦怠、萎靡、毛色枯稿无光泽渐呈黄色,甚至脱毛,裸露皮肤,粪便湿稀,量大;治疗组先出现精神倦怠、萎靡、毛色枯稿无光泽呈黄色,打绺,粪便湿稀,量大,继之排尿量逐渐减少,粪便量也逐渐减少。3组大鼠体重均增加,正常对照组增加最多,治疗组次之,模型对照组增加最少,P<0.05,差异具有统计学意义。正常对照组血糖比较稳定,第6周、第10周治疗组的血糖明显低于模型对照组(P<0.05),差异具有统计学意义。治疗组的SOD含量高于模型对照组,差异具有统计学意义(P<0.05);模型对照组的MDA高于治疗组,差异具有统计学意义(P<0.05)。病理切片显示,空白组为正常结构;模型对照组视网膜可见较多新生血管,细胞变形,排列紊乱;治疗组视网膜上新生血管较对照组明显减少,细胞排列较规则,差异具有统计学意义(P<0.05)。结论消朦灵方能减轻糖尿病大鼠部分相关症状,对血糖有一定程度的控制作用,还可以抑制糖尿病大鼠体内的自由基,改善体内氧化-抗氧化平衡状态,预防糖尿病视网膜病变的发生。
Objective To investigate the effect and mechanism of Xiaominling on diabetic rats. Methods Thirty male Sprague-Dawley rats were randomly divided into normal control group (n = 10) and model control group (n = 20). The rats were randomly divided into two groups: Warm water was given to the stomach to observe the effect of Xiao Meng Ling Decoction on the whole body state, body weight, blood glucose, SOD, MDA and the retina structure in rats. Results Compared with the normal control group, the model control group had mental fatigue, malaise, pale color and dullness, yellowing and even hair removal, bare skin and wet stool with a large amount of volume. The treatment group showed mental fatigue, malaise, Shiny yellow, hitting, stool wet thin, large volume, followed by gradually reduce the amount of urine output, stool volume also gradually reduced. The weight of rats in the three groups increased, the increase in the normal control group, the treatment group followed by the model control group increased least, P <0.05, the difference was statistically significant. The blood glucose in the normal control group was stable, and the blood glucose in the 6th week and the 10th week in the treatment group was significantly lower than that in the model control group (P <0.05). The difference was statistically significant. The content of SOD in the treatment group was higher than that in the model control group, the difference was statistically significant (P <0.05). The MDA in the model control group was higher than that in the treatment group. The difference was statistically significant (P <0.05). Pathological sections showed that the blank group was normal structure. The retina of the model control group showed more neovascularization, and the cells were deformed and disordered. The number of neovascularization on the retina of the treated group was significantly decreased compared with the control group, and the arrangement of the cells was regular (P <0.05). Conclusion Xiaomin Ling can relieve some of the symptoms of diabetic rats, has a certain degree of control of blood glucose, but also can inhibit free radicals in diabetic rats to improve the oxidation-oxidation balance in vivo and prevent the occurrence of diabetic retinopathy.