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目的:观察有机锗(β-羧乙基锗倍半氧化物,β-carboxyethylgermanium sesquioxide,Ge-132)对佐剂诱导关节炎大鼠炎症组织局部组胺(histamine,HA)及5-羟色胺(5-hydroxytryptamine,5-HT)表达的影响。方法:雄性SD大鼠随机分为6组(n=10):空白对照组,模型组,低(10mg/kg)、中(20mg/kg)、高剂量(40mg/kg)Ge-132治疗组以及吲哚美辛(IDMT,1mg/kg)对照组。除空白对照组外其余各组大鼠均在右后足跖皮下注射弗氏完全佐剂(Freund’s complete adjuvant,FCA)0.1ml制备实验性类风湿性关节炎(RA)大鼠模型。于FCA注射前、注射造模后第3、6、9、12、15、18、21天测大鼠体质量和右足足跖容积,计算体质量增长率和足跖肿胀率。造模第21天处死大鼠,取右后足,测定炎症组织局部HA及5-HT的含量。结果:造模第21天,中、高剂量Ge-132治疗组及IDMT对照组大鼠体质量增长率优于模型组及低剂量组(P<0.01),与空白对照组无统计学差异。低、中、高剂量组大鼠足跖肿胀程度明显低于模型组(P<0.01),但高于IDMT对照组(P<0.01或0.05);高剂量组肿胀程度明显低于低、中剂量组(P<0.05)。低、中、高剂量组及IDMT对照组大鼠关节炎局部HA含量均显著低于模型组(P<0.01);但低、中剂量组高于高剂量组、IDMT对照组(P<0.01)。中、高剂量组及IDMT对照组大鼠关节炎局部5-HT含量显著低于模型组和低剂量组(P<0.01)。结论:中、高剂量Ge-132可能通过抑制HA、5-HT等炎症介质的释放显著减轻关节炎大鼠的局部炎症,效果与IDMT相近。
OBJECTIVE: To observe the effects of organogermanium (β-carboxyethylgermanium sesquioxide, Ge-132) on histamine (HA) and 5-hydroxytryptamine -hydroxytryptamine, 5-HT) expression. Methods: Male SD rats were randomly divided into 6 groups (n = 10): blank control group, model group, low (10mg / And indomethacin (IDMT, 1 mg / kg) control group. Except the blank control group, experimental rats with rheumatoid arthritis (RA) were injected subcutaneously with 0.1 ml of Freund’s complete adjuvant (FCA). Before FCA injection, the body weight and right foot plantar volume of rats were measured on the 3rd, 6th, 9th, 12th, 15th, 18th and 21st day after injection. The body mass growth rate and plantar swelling rate were calculated. Rats were killed on the 21st day after modeling, and the right hind foot was taken to determine the content of HA and 5-HT in the inflammatory tissues. Results: On the 21st day, the body mass growth rate of Ge-132 treatment group and IDMT control group was better than that of the model group and the low-dose group (P <0.01), and no significant difference compared with the blank control group. The degree of paw edema in low, middle and high dose groups was significantly lower than that in model group (P <0.01), but higher than that in IDMT control group (P <0.01 or 0.05); the swelling degree in high dose group was significantly lower than that in low and middle dose groups Group (P <0.05). The content of HA in the low, medium and high dose groups and the IDMT control group were significantly lower than those in the untreated group (P <0.01), but were lower in the low and medium dose groups than in the high dose and IDMT control groups (P <0.01) . The content of 5-HT in middle and high dose group and IDMT control group was significantly lower than that in model group and low dose group (P <0.01). Conclusion: Ge-132 at medium and high doses may significantly reduce the local inflammation of arthritic rats by inhibiting the release of inflammatory mediators such as HA and 5-HT, which is similar to that of IDMT.