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为研究澳大利亚荒漠植物比拉底白刺的抗旱性,以比拉底白刺幼苗作为研究材料,在100 mmol/L甘露醇溶液模拟干旱的环境下,观察了植物形态变化,测定了植物的相对含水量(relative water content,RWC)、过氧化物酶活性(peroxidase,POD)、丙二醛(malondiadehyde,MDA)以及游离脯氨酸(proline,Pro)含量的动态变化。结果表明,干旱处理过程中,比拉底白刺叶片从基部往上逐渐萎蔫变黄,推测其通过保护顶芽的生命力以适应干旱环境;相对含水量前期相对稳定,后逐渐下降;POD活性先缓慢升高,后快速上升;游离Pro浓度总体呈上升趋势;MDA含量先升后降。差异显著性分析表明,与对照相比,POD和游离Pro的变化趋势与对照组相比差异显著,说明二者与比拉底白刺的抗旱性具有相关性,通过减少质膜过氧化保护细胞完整性;MDA含量变化不明显,可能由于POD和游离脯氨酸的作用使得白刺细胞的膜脂系统未遭受活性氧自由基的强烈攻击。
In order to study the drought resistance of Piraetilis in desert plants of Australia, taking Pirardona asiatica seedlings as experimental material, the morphological changes of plants were observed in a simulated drought environment of 100 mmol / L mannitol solution. (RWC), peroxidase (POD), malondiadehyde (MDA), and proline (Pro) contents in the leaves of the two groups were determined. The results showed that in the process of drought treatment, the leaves of Piraea tenuiflora gradually wilted and turned yellow from the basal part, suggesting that it could adapt to the drought environment by protecting the vitality of the terminal bud; the relative water content was relatively stable at the early stage and then decreased gradually; Slowly increased, then increased rapidly; the concentration of free Pro generally showed an upward trend; the content of MDA increased first and then decreased. Significant difference analysis showed that, compared with the control, POD and free Pro trends compared with the control group significant difference, indicating that the two with Piraea drought resistance is correlated, by reducing plasma membrane peroxidation to protect cells Integrity, and the content of MDA was not changed obviously. The membrane lipid system of the white needles was not attacked strongly by reactive oxygen species due to the action of POD and free proline.