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mPing是水稻中第一个被鉴定出的有活性的MITE类转座子,为了探索mPing在水稻粳稻品种日本晴和籼稻品种93-11基因组中的分布差异,本研究首先运用Southern杂交的方法初步检测m Ping在两个亚种中拷贝数的差异,然后通过同源性探寻方法发现,m Ping在水稻亚种日本晴和93-11基因组中拷贝数分别为52和14,并且日本晴基因组中的m Ping均为m Ping-1,93-11中m Ping-1的拷贝数为3,m Ping-2的拷贝数为11。通过分析m Ping上下游5 kb侧翼序列发现m Ping在日本晴和93-11中分别与23和3个已知基因相关联。本研究为阐明以m Ping的分布多样性为主要原因的粳稻和籼稻之间的遗传差异提供初步理论基础。
mPing is the first active MITE transposon in rice. In order to explore the distribution of mPing in the 93-11 genome of japonica rice varieties Nipponbare and Indica, we first detected the distribution of mPing by using Southern hybridization m Ping in two subspecies copy number differences, and then by homology search method found m Ping in rice subgenus Nipponbare and 93-11 genome copy number 52 and 14 respectively, and the Nipponbare genome m Ping All m Ping-1, the copy number of m Ping-1 in 1, 3-11 is 3, and the copy number of m Ping-2 is 11. By analyzing the upstream and downstream 5 kb flanking sequences of m Ping, m Ping was correlated with 23 and 3 known genes in Nipponbare and 93-11, respectively. This study provided the preliminary theoretical basis for clarifying the genetic differences between japonica and indica with m Ping distribution diversity as the main reason.