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目的在生长光强为1.5、10、30、55、100、270μmol/(m2.s)下对弱光生态型盾叶薯蓣的薯蓣皂苷元和生物量分别进行测定,以探讨不同光强与盾叶薯蓣中薯蓣皂苷元和生物量的关系,并找出弱光生态型盾叶薯蓣的最适生长光强。方法采用TLC法和RP-HPLC法测定其根状茎中薯蓣皂苷元的量。结果不同光强对盾叶薯蓣的薯蓣皂苷元的量有显著影响。在100、55μmol/(m2.s)的光强下,根状茎中薯蓣皂苷元的量最高,分别占其干质量的0.45%和0.55%。光照强度对盾叶薯蓣的生长有显著的影响,在30、55、100μmol/(m2.s)下长势良好,其中在100μmol/(m2.s)下生长最好。光照强度影响叶片的形状,随着光照强度的增加,叶片长与宽的比率也随之增加。在270μmol/(m2.s)的强光照射下,叶片长与宽的比率最大。光照强度能显著地影响植株的总叶面积,在100μmol/(m2.s)光强下植株总的叶面积最大,总的生物量最高,根状茎的生物量与整个植株生物量的比值最大。结论弱光型盾叶薯蓣是一种薯蓣皂苷元量较高的薯蓣变种,这将为该药源植物的选择育种以及栽培提供理论指导。
Aim To determine the diosgenin and biomass of Dioscorea opposita Dioscorea nipponica at light intensities of 1.5, 10, 30, 55, 100 and 270μmol / (m2.s), respectively, Leaf dioscorea diosgenin and biomass, and find out the optimum light intensity of low light ecotype Dioscorea zingiberensis. Methods The amount of diosgenin in rhizomes was determined by TLC and RP-HPLC. The results showed that different light intensity had a significant effect on the amount of diosgenin in Dioscorea zingiberensis. At the light intensities of 100 and 55μmol / (m2.s), the content of diosgenin in rhizomes was the highest, accounting for 0.45% and 0.55% of their dry mass, respectively. Light intensity had a significant effect on the growth of Dioscorea zingiberensis. Growing well at 30, 55 and 100μmol / (m2.s), the best growth was at 100μmol / (m2.s). The light intensity affected the shape of the leaves. With the increase of light intensity, the ratio of the length to the width of the leaves also increased. Under 270μmol / (m2.s) intense light, the ratio of leaf length to width was the largest. Light intensity could significantly affect the total leaf area of the plant, with the highest total leaf area under the light intensity of 100μmol / (m2.s), the highest total biomass, and the highest ratio of rhizome biomass to the whole plant biomass . Conclusion Low light type Dioscorea zingiberensis is a variety of diosgenin higher diosgenin varieties, which will provide theoretical guidance for the selective breeding and cultivation of the source plants.