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以废弃木薯渣为原料,采用生物酶结合碱法提取纤维素,通过单因素试验和正交试验,确定了木薯渣中纤维素提取的最佳工艺条件,并以扫描电镜对提取纤维素的表面结构进行了表征。结果表明,在超声波功率为105 W辅助下,提取木薯渣纤维素的最佳工艺条件为α-淀粉酶用量0.8%、酶解时间60 min、氢氧化钠浓度6%和碱解时间90 min,此条件下所得木薯渣纤维素产品含量为63.84%,是一种可行、高效的纤维素提取方法。纤维素产品表面结构的扫描电镜图显示,提纯后的纤维素比表面积增大较明显,有利于后期对木薯渣纤维素的改性制备。
Using waste cassava residue as raw material, cellulase was extracted by the method of biological enzyme combined with alkali method. The optimum extraction conditions of cellulose in cassava residue were determined by single factor test and orthogonal experiment. The surface of cellulose was extracted by scanning electron microscope The structure was characterized. The results showed that the optimal extraction conditions of cassava residue cellulose with the aid of ultrasonic power of 105 W were as follows: the dosage of α-amylase was 0.8%, the enzymolysis time was 60 min, the concentration of sodium hydroxide was 6% and the alkaline hydrolysis time was 90 min, Under these conditions, the content of cassava residue cellulose product is 63.84%, which is a feasible and efficient cellulose extraction method. Scanning electron micrographs of the surface structure of the cellulose product showed that the specific surface area of the cellulose increased more obviously, which was beneficial to the later preparation of the cellulose of cassava residue.