论文部分内容阅读
以化学农药氯唑灵诱变苎麻疫霉（ＰｈｙｔｏｐｈｔｈｏｒａｂｏｅｈｍｅｒｉａｅＳａｗａｄａ）的野生型菌株Ｅｃ－８获得异宗配合变异株，氯唑灵的有效诱变浓度为１０～３０μｇ／ｍＬ，以２０μｇ／ｍＬ处理３～８周效果最好。异宗配合变异株的交配型在单游动孢子或单菌丝片段后代均发生分离，出现了疫霉菌已知的全部５种交配型的个体。各交配型在单游动孢子无性系后代持续分离：Ａ１交配型菌株后代出现了Ａ１、Ａ２、Ａ０和Ａ１，Ａ２交配型，Ａ２交配型菌株后代出现了Ａ１、Ａ２、Ａ１Ａ２和Ａ０交配型，Ａ１，Ａ２交配型菌株后代出现了Ａ１、Ａ２、Ａ１Ａ２、Ａ０和Ａ１，Ａ２交配型，Ａ０交配型菌株后代出现了Ａ１、Ａ１Ａ２、Ａ０和Ａ１，Ａ２交配型，而部分回复为Ａ１Ａ２交配型的菌株后代也出现了Ａ１、Ａ２、Ａ１Ａ２、Ａ０和Ａ１，Ａ２５种交配型。８０％以上的变异株在１２°Ｃ、黑暗中贮存２个月后仍可保持其原有交配型，部分变异株在菌丝块移植继代培养７代后仍然保持异宗配合特性。对部分Ａ１交配型单孢株激素产生与接受类型的测定结果表明，Ａ１交配型变异株至少包括Ｓ１和Ｓ２两种性征类型。上述结果提示，疫霉属卵菌性别的产生可能是由同宗配合演化为异宗配合，并可能同时分化产生不?
The heterophile co-mutant strain was obtained by mutagenizing the wild-type strain Ec-8 of Phytophthora boehmeriae Sawada with chemical pesticides. The effective mutagenic concentration of clozapine was 10 ~ 30μg / mL, 8 weeks the best. The mating type of the heteroplasmic variant strains segregated in either single zoospores or single mycelial segments and all 5 mating types of individuals known to Phytophthora blight appeared. Each mating type continued to segregate in the progeny of the monoclone germ clones: A1, A2, A0 and A1, A2 mating type appeared in the A1 mating type, and A1, A2, A1 A2 and A0 mating type in the A2 mating type, A1, A2, A1A2, A0 and A1, A2 were found in the offspring of the mating type A1 and A2, and A1, A1A2, A0 and A1 and A2 were found in the offspring of A0 mating type, but partially recovered as A1A2 mating type Strains also appeared A1, A2, A1A2, A0 and A1, A25 kinds of mating type. More than 80% of mutants remained their original mating type after being stored at 12 ° C for 2 months in the dark. Some of the mutants remained heterotrimeric after subculture for 7 generations. The result of hormone production and acceptance type in the part A1 mating type single spore strain showed that the A1 mating variant at least includes both the S1 and S2 characterotypes. The above results suggest that the occurrence of Phytophthora Oocytogenes may be caused by the same clan evolved into heterogamy, and may not differentiate at the same time?