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益气滋肾冲剂是我们多年来治疗IgA肾病的有效方药,该药对IgA肾病的血尿和蛋白尿具有较好的效果。为了探讨该药的作用机理,本文应用小鼠IgA肾病模型,观察益气滋肾冲剂对小鼠IgA肾病肾小球免疫复合物沉积的影响。 实验材料 1、主要试剂 FTTC-兔抗小鼠IgA抗体为美国ZYMED公司产品,批号:50525148RR(日本广岛大学医学部赠送);FITC-羊抗小鼠IgG抗体为军事医学科学院产品,批号:9606;FTTC-兔抗小鼠C_3抗体为MBL产品,批号071;牛血清蛋白(BSA)为德国PROMEGA公司产品,北京华美公司分装,批号:BP0024;其他试剂为国产分析纯。 2、实验动物 雄性昆明小鼠150只,体重18-22克,购自北京医科大学实验动物中心,尿检蛋白和潜血全部为阴性,经5天适应性饲养后,用于实验。
Yiqi Zishen Granule is an effective prescription for treating IgA nephropathy for many years. It has a good effect on hematuria and proteinuria in IgA nephropathy. In order to investigate the mechanism of action of the drug, the mouse IgA nephropathy model was used to observe the effect of Yiqi Zishen Granules on the deposition of glomerular immune complexes in mice with IgA nephropathy. Experimental materials 1. The main reagent FTTC-rabbit anti-mouse IgA antibody is a US ZYMED product, batch number: 50525148 RR (presented by the medical department of Hiroshima University, Japan); FITC-goat anti-mouse IgG antibody is a product of the Academy of Military Medical Sciences, lot number: 9606; FTTC - Rabbit anti-mouse C_3 antibody was MBL product, lot number 071; bovine serum albumin (BSA) was product of German PROMEGA company, Beijing Huamei company was loaded separately, batch number: BP0024; other reagents were domestically produced analytically pure. 2. Experimental Animals 150 male Kunming mice, weighing 18-22 g, were purchased from the Experimental Animal Center of Beijing Medical University. The urine test proteins and occult blood were all negative. After 5 days of adaptive rearing, they were used for experiments.