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应用DNA重组技术将编码人碱性成纤维细胞生长因子(bbFGF)的基因克隆至原核高效表达质粒pBV_(221)的启动子下游。SDS-SAGE、ELISA和NTT活性监测结果表明:该重组质粒pBV-hbFGF在大肠杆菌DH5α中,经42℃诱导后,可表达出有较高生物活性的hbFGF。
The gene encoding human basic fibroblast growth factor (bbFGF) was cloned downstream of the promoter of prokaryotic expression plasmid pBV_ (221) using DNA recombinant technology. The results of SDS-SAGE, ELISA and NTT assay showed that the recombinant plasmid pBV-hbFGF could express hbFGF with high biological activity in E. coli DH5α induced at 42 ℃.