目的：探讨芪术汤的抗氧化作用.方法：芪术汤由黄芪、白术、三七和茯苓四种中药组成.对芪术汤全方研究的同时，进行该方的拆方研究及单味药研究，以DPPH、氧自由基清除活性以及药物对鼠肝组织匀浆中TBARS形成的抑制等项实验作为检测指标，进行体外抗氧化活性观察.同时，复制大鼠脑缺血/再灌注损伤的动物模型，观察芪术汤在预防和治疗脑缺血/再灌注损伤中的作用.结果：芪术汤及其三种不同形式的组方、部分单味药都具有较好的体、内外抑制TBARS的形成，提高GPX酶活性的功能，但芪术汤全方比其他形式的组方及单味药具有更高的抗氧化活性.结论：芪术汤是良好的抗氧化损伤天然药物，对脑缺血/再灌注损伤具有有效的预防和治疗作用.“,”AIM: To study the antioxidant activity of Qizhu Tang ( QZT) both in vivo and in vitro. METHODS: QZT consists of 4 herbal constituents (Rhizoma Atractylodis Macrocephalae, Poria cocos, Radix Notoginseng, and Radix Astragali), each of the components and their combinations were examined in vitro for 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical scavenging activities, and for the inhibition of thiobarbituric acid-reactive substances (TBARS) formation in rat liver homogenate. At the same time, their in vivo protective effect on cerebral ischemia-areperfusion injury was determined in rats. RESULTS: Only the preparations having a higher antioxidant activity comparable to QZT in all three in vitro assays were relatively active in vivo both for TBARS inhibition and glutathione peroxidase preservation, although the activities were much lower than that of QZT as a whole. CONCLUSION: QZT formula is a good natural antioxidant having an effective preventive effect against cerebral ischemia repeffusion damgge.